By Prof. Dr. Toni Lindl, Dr. Rosemarie Steubing(auth.)
The 1st atlas in lots of years giving researchers a very good visible reference of the prestige in their telephone strains. Given the expanding value of good outlined mobile types particularly in biomedical examine it is a sorely wanted source for everybody appearing telephone culture.Content:
Chapter 1 creation (pages 1–4):
Chapter 2 simple cellphone tradition strategies (pages 5–9):
Chapter three checklist of phone traces and Human fundamental Cells (in Alphabetical Order) (pages 11–14):
Chapter four telephone traces and Human fundamental Cells (pages 15–492):
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Extra resources for Atlas of Living Cell Cultures
102, 77–81. CHT04AAC 03/20/2013 22:1:1 Page 44 BT-20, 100Â Leica. BT-20, 100Â Leica. BT-20, 400Â Leica. Atlas of Living Cell Cultures, First Edition. Toni Lindl and Rosemarie Steubing. # 2013 Wiley-VCH Verlag GmbH & Co. KGaA. Published 2013 by Wiley-VCH Verlag GmbH & Co. KGaA. CHT04AAC 03/20/2013 22:1:2 Page 45 Human Cell Lines BT-20 B Origin and General Characteristics Organism: Ethnicity: Age: Gender: Tissue: Cell type: Morphology: Growth properties: Description: Homo sapiens (human) Caucasian 74 years Female Breast Mammary gland Epithelial Monolayer BT-20 was established by Lasfargues and Ozzello in 1958 by isolation and cultivation of cells spilling out of the tumor when it was cut into thin slices.
02% EDTA solution. 02% EDTA solution and let the culture sit at 37 C until the cells detach. J. et al. (1973) In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. , 51, 1417–1413. CHT04AAC 03/20/2013 22:0:52 Page 26 A-427, 100Â Leica. A-427, 100Â Leica. A-427, 400Â Leica. Atlas of Living Cell Cultures, First Edition. Toni Lindl and Rosemarie Steubing. # 2013 Wiley-VCH Verlag GmbH & Co. KGaA. Published 2013 by Wiley-VCH Verlag GmbH & Co.
51, 1417–1423. CHT04AAC 03/20/2013 22:0:54 Page 30 A-498, 100Â Leica. A-498, 100Â Leica. A-498, 400Â Leica. Atlas of Living Cell Cultures, First Edition. Toni Lindl and Rosemarie Steubing. # 2013 Wiley-VCH Verlag GmbH & Co. KGaA. Published 2013 by Wiley-VCH Verlag GmbH & Co. KGaA. J. 0 mM sodium pyruvate, 1% nonessential amino acids 90%, fetal bovine serum (FBS) 10%; G6PD, B. 02% trypsin EDTAsolution and incubate for 5–10 min at 37 C. Stop with double volume of fresh medium when cells are detached.